FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n02_ds395-041-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_ds395-041-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9564164
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3469913.62803272716779Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2535252.6507805595972633No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA85850.056.82953319
ACACGAG87850.055.5758820
AGAGCAC314750.055.26457215
AAGGGGG445600.055.10974567
AAAGGGG447350.054.9408166
GAGCACA309100.054.72379716
CACGAGA90150.053.45580321
GCACACG208600.053.0690218
CACGTAG69500.052.11733621
AGGGGGG476200.051.81504468
AGCACAC233250.051.52726417
ACACGTA70750.050.60612520
AAAAGGG491450.050.2246265
AAGAGCA428400.049.8434114
GCACAAG91200.047.66316618
CACACGT136500.044.4342219
ACGAGAT110500.043.89626722
AGCACAA100900.043.11599317
AAAAAGG583500.042.51957764
GAGCAAG85250.040.76742616
Produced by FastQC (version 0.11.2)