FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n02_ds395-041-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_ds395-041-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9445783
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3935844.166769446217429Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3235053.424861655195763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA288450.060.7396716
AGAGCAC294550.060.63466315
GCACACG187100.060.40099318
AGCACAC206900.059.2048617
ACACGAG82400.057.34073620
CACACGA83250.056.75347519
AAAGGGG492950.056.6943166
AAGGGGG496700.056.23809467
CACACGT116700.055.48093419
AAGAGCA403550.054.51693714
AGGGGGG521550.053.79937468
CACGTAG65100.053.54472421
CACGAGA89300.053.29982421
ACACGTA65500.052.5253520
AAAAGGG546900.051.2170465
GCACAAG96300.050.33537718
ACACGTC63950.046.46477520
AGCACAA105650.046.11259517
ACGTCAG56150.045.87397822
ACGAGAT108000.043.84422322
Produced by FastQC (version 0.11.2)