FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n02_ds395-032-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_ds395-032-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11342631
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3651483.2192530992148116Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1034450.9120018098093821No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA401000.056.93236516
CACACGA118150.056.78618219
AGAGCAC411100.056.62361515
ACACGAG120400.055.43478420
GCACACG265100.055.13265618
AGCACAC294250.054.19077717
CACGAGA123700.053.2469421
AAGGGGG487800.053.02530767
AGGGGGG497950.052.9384568
CACGTAG95500.052.77203821
AAAGGGG493300.052.42700666
AAGAGCA565850.052.2343814
ACACGTA98650.050.66280720
GCACAAG127050.048.6490318
CACACGT166150.046.9320119
AAAAGGG554700.046.77587565
GAGCAAG119800.045.7207916
AGCACAA136450.045.3489117
ACGAGAT147100.044.58632322
AGAGAGA209100.043.20083215
Produced by FastQC (version 0.11.2)