FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n02_ds395-032-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_ds395-032-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10676523
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5285244.950338232774847Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3511233.288739227180984No Hit
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT107840.10100666668352608Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC336400.061.41320415
GAGCACA332300.061.1282616
GCACACG207800.060.9856918
AGCACAC231200.059.68757217
ACACGAG92650.059.04137820
CACACGA93200.058.84316319
AAAGGGG643250.057.9105766
AAGGGGG646600.057.65383567
CACGTAG73450.056.70143521
CACACGT128300.055.04747819
AGGGGGG683400.054.8347868
AAGAGCA474500.054.65463314
CACGAGA100050.054.63900821
ACACGTA76050.054.53333720
AAAAGGG705050.053.02338465
GCACAAG113350.051.90284318
AGCACAA123050.047.81135617
GAGCAAG101850.046.01130316
ACGTCAG53900.045.84107622
ACGAGAT123250.044.2688322
Produced by FastQC (version 0.11.2)