FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n02_787.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_787.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6958854
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1478862.125148767311399No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC282750.406316902179583Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG44250.041.36612718
AGAGCAC68200.039.5661115
GAGCACA65950.039.2711316
CACACGA17850.039.01828419
ACACGAG18400.037.2821520
CACGTAG18600.036.87967721
AGCACAC55850.036.1583917
ACACGTA19500.035.53802520
CACGAGA20350.034.05216621
AAAGGGG60400.033.89566866
CACACGT35100.031.40919519
AAGAGCA113650.028.54720914
CCGGGTC5250.027.3447881
AAGGGGG80850.025.79832567
CCGTATC183600.025.35335455
CGCCGTA190550.024.5204753
CGTATCA191650.024.45277856
GCCGTAT194350.024.07705354
GTATCAT197700.023.7221857
TCGCCGT199450.023.21572552
Produced by FastQC (version 0.11.2)