FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n02_786.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_786.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4983656
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1008652.0239157758882236No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC193350.3879681904208477Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18600.035.18624518
AAAGGGG45900.033.8519866
AGAGCAC29650.032.81439215
CACACGA8100.032.40563619
GAGCACA29450.031.73001116
AGCACAC25150.028.24895117
AAGGGGG56300.027.66084167
ACACGAG9400.027.17990920
GGGGGAT12950.026.4993931
CACGAGA9650.026.11151121
CGGGGTG6050.024.8881571
CACACGT15850.024.06808319
GGGGAAC10300.023.7953742
CGCCGTA108600.023.71996553
CCGTATC107850.023.69020355
GCCGTAT111600.023.14505654
AAAAGGG68500.022.68329665
GGGGTTA14150.022.5173172
CGTATCA114600.022.50884856
TCGCCGT113700.022.37896552
Produced by FastQC (version 0.11.2)