FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n02_776.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_776.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7138858
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1311511.8371425793873475No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC160140.22432159317358602Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG29300.036.7911718
AGAGCAC45050.036.3587115
GAGCACA43900.036.27499416
AGCACAC35250.033.16254417
CACACGA14150.030.9175419
ACACGAG14300.030.59387420
AAAGGGG44450.028.894366
CACGAGA17150.025.71275721
CACGTAG13800.025.10720421
CACACGT25200.024.86015519
CCGTATC110300.023.06801655
AAGGGGG56850.022.83817367
ACACGTA14800.022.70230720
CGCCGTA111850.022.68576253
GCCGTAT113650.022.41884654
CCGGGTG5900.021.9593831
AAGAGCA94150.021.74668114
CGTATCA119750.021.42312856
TAGATCG78450.021.3717236
TCGCCGT120900.020.95866452
Produced by FastQC (version 0.11.2)