FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n02_163a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_163a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6498983
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1545512.378079770327142No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC414410.6376536144193637Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA24850.044.36581419
ACACGAG24300.044.36243420
GCACACG70300.043.0657218
GAGCACA92500.043.05941416
AGAGCAC94750.043.03392415
AGCACAC78400.041.74167317
AAAGGGG78450.039.746566
CACGAGA27600.039.1823321
CACGTAG22500.039.04221
AAGGGGG84050.037.01503867
ACACGTA24350.035.35957720
AAGAGCA135200.035.0255814
CACACGT53250.034.37528619
AGGGGGG100050.031.97250668
ACGTCAG25100.031.79084222
GTGACTG26750.030.3705331
TCGCCGG8100.030.2457270
CGGGGAC5050.029.1236721
ACGAGAT38850.027.38567522
TGACTGG29100.027.3120462
Produced by FastQC (version 0.11.2)