FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n01_ds774-041-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_ds774-041-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12216178
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1686401.3804644955238865TruSeq Adapter, Index 12 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG306700.040.52439570
CTGCTTG350000.036.5018758
TGCTTGA351600.036.43546359
ATGCCGG26650.036.3799670
GCTTGAA371100.034.80386760
GACGTGT138450.033.6575161
AAAAAAG397700.031.7092969
CTTGAAA412100.031.61300861
TCTGCTT410850.031.22345757
CCGTCTT418950.030.69384251
CTTCTGC432750.029.84567355
CGTCTTC431500.029.83356752
GTCTTCT434950.029.58912753
TGAAAAA451700.029.05826463
TGCCGTC441950.029.02531249
TTGAAAA457950.028.57761262
GCCGTCT450250.028.50556650
TTCTGCT451550.028.48667756
TCTTCTG456800.028.18238354
GAAAAAA488150.027.18236564
Produced by FastQC (version 0.11.2)