FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n01_ds774-041-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_ds774-041-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13040838
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG4200293.2208743027096878TruSeq Adapter, Index 8 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATG212140.16267359505577786TruSeq Adapter, Index 8 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG628400.054.0538670
GACGTGT110750.045.1144641
AAAAAAG771200.044.63944669
CTTGAAA886000.036.91726761
TGAAAAA965850.036.6479263
GCTTGAA885300.036.5748260
TTGAAAA902450.036.51194862
TGCTTGA863400.036.1770559
CTGCTTG873550.035.071458
GAAAAAA1019300.035.0316564
TCTGCTT957800.032.3699157
CTTCTGC973200.031.44732755
TTCTGCT946550.031.27911656
ATGCCGG146850.030.6264470
GTCTTCT974100.030.37938953
CGTCTTC1004800.030.25583852
TCTTCTG991700.030.2042254
ACGTGTG191600.029.9149821
CCGTCTT1047350.029.6014451
GCCGTCT1066650.028.75399450
Produced by FastQC (version 0.11.2)