FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n01_ds774-032-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_ds774-032-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17494
Sequences flagged as poor quality0
Sequence length76
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATG386922.116154109980567TruSeq Adapter, Index 14 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA710.40585343546358754TruSeq Adapter, Index 14 (97% over 38bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTAT620.3544072253344004TruSeq Adapter, Index 14 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCAGCTCGTATG570.32582599748485197TruSeq Adapter, Index 14 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC440.2515148050760261TruSeq Adapter, Index 14 (98% over 50bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTAT440.2515148050760261TruSeq Adapter, Index 14 (97% over 48bp)
AGAATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTAT360.2057848405167486TruSeq Adapter, Index 14 (97% over 39bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCC360.2057848405167486TruSeq Adapter, Index 14 (100% over 39bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTAT340.19435234937692925TruSeq Adapter, Index 14 (97% over 48bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTC290.16577112152738083TruSeq Adapter, Index 14 (100% over 38bp)
AGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC280.16005487595747114TruSeq Adapter, Index 14 (97% over 37bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC260.14862238481765175TruSeq Adapter, Index 14 (97% over 46bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCC240.1371898936778324TruSeq Adapter, Index 14 (98% over 50bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTT240.1371898936778324TruSeq Adapter, Index 8 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATGTCGTATG230.13147364810792272TruSeq Adapter, Index 14 (97% over 41bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC190.10860866582828399TruSeq Adapter, Index 14 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG190.10860866582828399TruSeq Adapter, Index 8 (100% over 49bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTA180.1028924202583743TruSeq Adapter, Index 14 (97% over 47bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCGCGTATG180.1028924202583743TruSeq Adapter, Index 14 (97% over 42bp)
CGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTC180.1028924202583743Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGA150.002188266570.0000168
AACTCAA150.002188266570.0000123
ATCTGGC150.002188266570.0000117
TCTGGGT207.7122044E-570.018
AAAAAGG4450.065.280970
AAAAAAG4600.063.15217269
GAAAAAA5600.055.00000464
TGAAAAA5650.054.51327563
TTGAAAA5700.053.42105562
GTATGGC200.00683842952.570
TCAAGAT200.00683842952.524
GCGGATG200.00683842952.570
CGCGGAT200.00683842952.569
ATCTGAA200.00683842952.517
CTTGAAA5900.051.6101761
GACGTGT3300.049.8484841
GCTTGAA6150.049.51219660
AGACGTG950.047.894741
ATGCCGG451.5916994E-646.66666470
TGCTTGA6700.044.92537359
Produced by FastQC (version 0.11.2)