FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n01_ds774-032-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_ds774-032-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11946344
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG127806310.698360937873545TruSeq Adapter, Index 9 (100% over 49bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA253170.21192257648030227TruSeq Adapter, Index 9 (100% over 38bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG205330.17187685203104816TruSeq Adapter, Index 9 (100% over 39bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC164660.13783296379210241TruSeq Adapter, Index 9 (100% over 46bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT158330.1325342715729599TruSeq Adapter, Index 9 (100% over 48bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC153750.12870046266874619TruSeq Adapter, Index 9 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG124950.10459266868591763TruSeq Adapter, Index 9 (100% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC122470.10251671975961851TruSeq Adapter, Index 9 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG1450150.062.35276470
ATGCCGG54300.058.33453870
AAAAAAG1594500.057.0943169
GACGTGT149650.049.1078451
TGAAAAA2209150.043.45512463
TTGAAAA2217800.043.2151662
GAAAAAA2235700.043.06883664
CTTGAAA2296300.041.67076561
GCTTGAA2523150.037.84256460
AAAAGGG125600.036.9235370
TGCTTGA2614850.036.44050259
ACGTGTG312500.035.8857571
CTGCTTG2710700.035.1245658
TCTGCTT2783900.034.23102657
GAATCGG25250.033.8213462
TTCTGCT2824600.033.72401456
CTTCTGC2846450.033.52989255
TCTTCTG2907850.032.80037354
GTCTTCT2946050.032.38599853
CGTCTTC3148400.030.31564152
Produced by FastQC (version 0.11.2)