FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n01_ds395-041-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_ds395-041-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9564164
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG3208663.3548776453435973TruSeq Adapter, Index 10 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC96390.10078246253410125TruSeq Adapter, Index 10 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG447750.053.54648670
AAAAAAG525650.046.07736669
GCTTGAA594500.040.40781460
ATGCCGG64950.040.2547870
CTTGAAA602550.040.23912461
TGCTTGA592450.039.7323159
TGAAAAA634700.039.7057763
GAAAAAA639300.039.5295764
TTGAAAA619750.039.2915962
CTGCTTG598800.039.0124458
GACGTGT115250.038.828031
TCTGCTT639100.036.71124357
TTCTGCT641450.035.78551556
CTTCTGC653000.035.64551555
GTCTTCT655250.035.2164253
TCTTCTG684300.033.8769854
CCGTCTT708550.033.43174451
CGTCTTC707100.033.2528152
GCCGTCT732650.032.07372750
TGCCGTC770800.030.9995349
Produced by FastQC (version 0.11.2)