FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n01_ds395-041-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_ds395-041-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9445783
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG3658363.8730087278100713TruSeq Adapter, Index 5 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG504800.054.31020770
AAAAAAG590300.046.8650569
ATGCCGG54050.046.753570
GACGTGT89000.042.9608761
CTTGAAA724150.038.8462961
GCTTGAA736750.037.9966660
TGAAAAA756500.037.88355663
TTGAAAA754800.037.39407362
GAAAAAA769200.037.39003464
TGCTTGA749500.036.8599459
CTGCTTG762000.036.1588258
TCTGCTT811950.034.0807757
TTCTGCT818350.033.373756
CTTCTGC825000.033.3592455
GTCTTCT847550.032.3015153
TCTTCTG858750.032.0033554
CCGTCTT904800.030.60195451
CGTCTTC906050.030.4515752
GCCGTCT936850.029.43534350
TGCCGTC968150.028.68615349
Produced by FastQC (version 0.11.2)