FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n01_ds395-032-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_ds395-032-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11342631
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG3618393.1900799735087917TruSeq Adapter, Index 11 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG483250.054.0598670
AAAAAAG545500.048.3591869
ATGCCGG48850.046.50024870
GACGTGT112200.040.7526321
GAAAAAA739400.037.95520464
CTTGAAA739850.037.69557661
GCTTGAA739250.037.54641760
TGAAAAA746650.037.5350963
TTGAAAA750200.037.18484562
TGCTTGA747700.036.86478859
CTGCTTG762400.036.1492458
TCTGCTT800450.034.46146857
TTCTGCT815100.033.75190756
CTTCTGC823250.033.5110155
GTCTTCT842700.032.75774453
TCTTCTG859300.032.0969854
ACGTGTG200150.030.8741111
CCGTCTT951950.028.99464451
CGTCTTC955550.028.91837352
GCCGTCT1018100.027.08312650
Produced by FastQC (version 0.11.2)