FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n01_ds395-032-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_ds395-032-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10676523
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG5249024.916413330444752TruSeq Adapter, Index 6 (100% over 49bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA109660.10271134151071469TruSeq Adapter, Index 6 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG661700.055.3805570
ATGCCGG38100.049.69858670
AAAAAAG774350.047.70364469
GACGTGT84000.041.635691
CTTGAAA964200.040.02494461
TGAAAAA980400.039.69202463
GAAAAAA1000350.039.1313764
TTGAAAA990700.039.0673862
GCTTGAA1008300.038.11487260
TGCTTGA1036450.036.91757659
CTGCTTG1066900.035.8311258
TCTGCTT1120500.034.17301657
CTTCTGC1147300.033.46018255
TTCTGCT1143600.033.45213356
GTCTTCT1192700.032.21541653
TCTTCTG1196350.032.08539654
ACGTGTG152850.030.7831731
CCGTCTT1268250.030.31827251
CGTCTTC1277150.030.11795452
GCCGTCT1326400.028.93883350
Produced by FastQC (version 0.11.2)