FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n01_787.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_787.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6958854
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG279360.401445410408093TruSeq Adapter, Index 4 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG159350.028.92699264
TGCTTGA161600.028.50236965
GCTTGAA170850.027.04116466
CCGTCTT180750.025.482857
TGCCGTC185500.024.8868855
GCCGTCT186650.024.71479456
CGTCTTC188850.024.51954558
CTTGAAA197950.023.76348967
GACGTGT29350.023.6198141
CTTCTGC195650.023.57789861
ATGCCGT197700.023.4042354
TCTGCTT201700.023.07890963
GTCTTCT202850.022.91356359
TCTTCTG204550.022.56913260
TATGCCG205250.022.56004553
ACGTGTG42250.021.5459691
ATGCCGG8500.021.41153370
GTATGCC216500.021.37159252
TTCTGCT220800.021.09835262
TTGAAAA241700.019.66480368
Produced by FastQC (version 0.11.2)