FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n01_786.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_786.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4983656
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG188560.37835677261833484TruSeq Adapter, Index 3 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG94500.026.7037664
TGCTTGA96500.026.15005365
GCTTGAA104200.024.58713766
GACGTGT16850.024.3100381
CCGTCTT106950.023.7917857
TGCCGTC107750.023.68010355
GCCGTCT107950.023.50653856
CGTCTTC111700.022.87404658
ATGCCGT113550.022.59384554
TATGCCG116600.022.1226953
GTATGCC121400.021.27681752
CTTCTGC120000.021.26254761
TCTGCTT122750.020.90024863
GTCTTCT127150.020.3698959
TCTTCTG127800.019.96503360
CTTGAAA129750.019.93433267
ACGTGTG21500.019.859712
CGTATGC130300.019.63549851
CATCTCG128250.019.29419546
TTCTGCT135850.018.85907762
Produced by FastQC (version 0.11.2)