FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n01_776.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_776.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7138858
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG156880.21975503644980754TruSeq Adapter, Index 2 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG92700.025.82601264
TGCTTGA99300.024.35604365
CCGTCTT109300.022.6084957
TGCCGTC109200.022.56493255
GCCGTCT110450.022.43647256
GACGTGT26350.021.7911971
ATGCCGT115350.021.3618654
TATGCCG117050.021.26047953
GCTTGAA116450.021.06960766
CGTCTTC119950.020.7762358
GTATGCC127650.019.57727852
TCTGCTT128350.019.11626863
CTTCTGC131650.018.7967461
GTCTTCT134100.018.66226259
ACGTGTG31900.018.3222752
CGTATGC137550.018.21911651
TCTTCTG142350.017.65444260
TATCTCG140300.017.28810546
CTCGTAT142100.017.14324849
CTTGAAA151250.016.68467767
Produced by FastQC (version 0.11.2)