FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n01_163a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_163a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6498983
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG406940.6261595083415359TruSeq Adapter, Index 1 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT48550.026.032791
GCTTGAA215900.025.82511166
TGCTTGA224300.024.60829265
CTTGAAA232900.024.07532167
CTGCTTG232950.023.70973664
AAAAAGG131050.023.2089370
TCTGCTT268950.020.73130463
ACGTGTG63400.020.4248122
TGAAAAA281150.020.37931869
TTGAAAA282550.020.0925368
CTTCTGC281700.019.74313561
TTCTGCT286100.019.41518262
CCGTCTT290600.019.2349857
GCCGTCT294350.018.90668956
GTCTTCT298300.018.77381359
CGTCTTC298950.018.75640958
GAAAAAA312050.018.54048370
GTGACTG18000.018.4779511
TGCCGTC301000.018.454155
TCTTCTG303400.018.33133560
Produced by FastQC (version 0.11.2)