FastQCFastQC Report
Thu 20 Feb 2020
H2JHGAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JHGAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3773535
Sequences flagged as poor quality0
Sequence length151
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATAA742550.0141.75457
TGTCATA202150.0141.13526
GTTCATA182350.0139.833886
ATTCATA183950.0137.201546
TTTCATA282100.0136.711916
AGTCATA132350.0134.873766
GGTCATA162400.0132.952066
TATCATA210350.0131.952736
GTCATAA599150.0130.988547
CTTTCAT52150.0130.839745
TCATAAG2295950.0130.251658
CGTTCAT35950.0129.820435
ATGTCAT59300.0129.096135
GTGTCAT55200.0128.329325
GATCATA127800.0128.012396
CTTCATA130400.0127.457596
CGGTCAT29150.0127.0906755
TATTCAT70750.0127.021565
GCTCATA83350.0126.397376
CTGTCAT39300.0124.46195
Produced by FastQC (version 0.11.5)