FastQCFastQC Report
Thu 20 Feb 2020
H2JHGAFX2_n01_1751_tninsertions2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JHGAFX2_n01_1751_tninsertions2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3035401
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA202400.0141.261586
TGTCATA267450.0140.514136
TTCATAA948950.0140.217297
GTTCATA240200.0139.825676
TTTCATA345600.0139.239076
TATCATA246050.0135.028096
CGGTCAT33650.0133.999425
ATTCATA227150.0133.242086
AGTCATA174000.0132.8216
GTGTCAT70000.0131.316365
AGGTCAT57150.0130.652775
TCTCATA176350.0130.639976
TTGTCAT99200.0130.151725
GTTTCAT88350.0129.72485
CTTCATA166450.0128.915626
CTGTCAT52400.0128.800065
CGATCAT29050.0128.017365
CGTTCAT45800.0126.784075
TGTTCAT97200.0126.564895
ATGTCAT71900.0126.03145