FastQCFastQC Report
Thu 20 Feb 2020
H2JHGAFX2_n01_1747_tninsertions2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JHGAFX2_n01_1747_tninsertions2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958333
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCATA103900.0142.399846
GGTCATA64300.0141.824666
TGTCATA84250.0141.281516
GTTCATA71950.0140.749776
TTCATAA289400.0139.946557
TATCATA79400.0138.225276
AGTCATA53450.0135.758946
CGGTCAT11200.0135.272755
GTGTCAT20850.0134.89875
AGGTCAT16350.0134.783865
ATTCATA67500.0133.382816
TTGTCAT29850.0132.838845
TCTCATA54650.0132.512666
GTTTCAT28400.0132.474145
TGTTCAT28600.0132.308125
CGTTCAT13900.0131.421925
GCGGTCA2650.0131.303854
GGGTCAT18600.0130.950885
AGTTCAT16650.0130.178425
CTGTCAT15150.0130.148255
Produced by FastQC (version 0.11.5)