FastQCFastQC Report
Thu 20 Feb 2020
H2JHGAFX2_n01_1744_tninsertions2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JHGAFX2_n01_1744_tninsertions2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2831042
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATAA882400.0140.920447
GGTCATA187500.0140.777796
TGTCATA246750.0140.056326
TTTCATA334100.0137.983476
GTTCATA223850.0137.964466
TATCATA226050.0135.916186
ATTCATA207050.0135.468466
AGTCATA161000.0134.320456
TCTCATA167850.0131.646216
CGGTCAT32900.0131.550695
GTCATAA746400.0129.204427
CTTCATA153350.0128.871486
TCAGAAG144450.0128.32548
TTGTCAT93200.0126.9459845
GTTTCAT93850.0126.298515
CGTTCAT44800.0125.735455
CAGAAGT141950.0124.916699
ATGTCAT69100.0124.6387565
GCTCATA115300.0124.368886
AGGTCAT51700.0124.238995
Produced by FastQC (version 0.11.5)