FastQCFastQC Report
Thu 20 Feb 2020
H2JHGAFX2_n01_1734_tninsertions2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JHGAFX2_n01_1734_tninsertions2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922666
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA201850.0142.334846
TGTCATA255450.0141.445136
TTCATAA897300.0141.427197
TTTCATA335300.0141.079156
GTTCATA229750.0140.417226
ATTCATA205350.0138.284886
TATCATA237700.0137.428836
CGGTCAT38150.0135.871195
CCTATAG63750.0134.64182145
AGGTCAT50900.0133.028585
TGGTCAT72600.0132.310755
TTGTCAT93050.0132.215185
GTTTCAT86350.0132.063325
TCTCATA169450.0131.858146
GTGTCAT64350.0131.47355
AGTCATA166450.0130.57616
CGTTCAT45000.0130.332245
CTTCATA148600.0130.210396
ATTTCAT91350.0129.358495
ATGTCAT72150.0129.01635
Produced by FastQC (version 0.11.5)