FastQCFastQC Report
Thu 20 Feb 2020
H2JHGAFX2_n01_1657_2_tninsertions2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JHGAFX2_n01_1657_2_tninsertions2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3462788
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA236200.0141.608666
GTTCATA269600.0141.30096
TTTCATA385500.0141.186136
TGTCATA301400.0140.799656
TTCATAA1063700.0140.0997
TATCATA276600.0137.620126
CGGTCAT40150.0135.9575
TTGTCAT109150.0134.623895
CCGGTCA8150.0134.310964
GTGTCAT77900.0134.189935
ATTCATA248550.0133.609916
AGTCATA204800.0133.232996
GTTTCAT99350.0132.507395
GGGTCAT65850.0132.214585
AGGTCAT61950.0132.112755
TGGTCAT85250.0132.059365
CGTTCAT52900.0131.55515
TGTTCAT102450.0130.125345
TTTTCAT149300.0129.204365
ATTTCAT103550.0129.093065
Produced by FastQC (version 0.11.5)