FastQCFastQC Report
Thu 20 Feb 2020
H2JHGAFX2_n01_1657_1_tninsertions2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JHGAFX2_n01_1657_1_tninsertions2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2503647
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATAA772400.0142.337177
GGTCATA168350.0140.895926
TGTCATA220150.0139.257056
TCATAAG2304450.0139.104458
TTTCATA293050.0138.901286
GTCATAA617850.0138.694967
GTTCATA202550.0138.616046
AGTCATA136800.0138.309716
ATTCATA177450.0137.183826
TATCATA200050.0135.92736
TCTCATA145250.0134.20636
CTTCATA129000.0133.185476
CGGTCAT30850.0132.767415
GATCATA125050.0132.522846
TCAGAAG124800.0132.320978
CTCATAA393350.0132.212287
CCTCATA62500.0131.531956
ATCATAA576500.0130.611187
CAGAAGT121600.0130.318519
CCTATAG48650.0129.33807145
Produced by FastQC (version 0.11.5)