FastQCFastQC Report
Wed 19 Feb 2020
H2JCLAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JCLAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11409523
Sequences flagged as poor quality0
Sequence length36
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3773203.307062004257321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCC5050.019.01753410
GTAGGGA1400.018.22921819
AATGGGC5650.017.7943659
CCGAGGA6950.017.30402829
CGTAAGG2550.017.07010513
AGTGACT4750.016.43704817
CCTGCCG7150.016.35208125
GATCGCT6000.015.7623622
GAGGGTT7450.015.71508413
GGGCCAA6150.015.61794912
TAGTGAC5600.015.54181716
TTGGACA5650.015.43442630
TGGGCCA6350.015.36110711
TTGCCCT7250.015.32249421
CTAAGGG3150.015.243889
TCCGTGA5800.015.2357277
TCGTTAC4550.015.1633888
GGTCCCC1206.961818E-814.9872341
TAAGGTC4200.014.9784166
AGATCGC6650.014.8990321
Produced by FastQC (version 0.11.5)