FastQCFastQC Report
Wed 19 Feb 2020
H2JCLAFX2_n02_males.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JCLAFX2_n02_males.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84372225
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4478850.5308441255401288No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT2888190.34231525836849747Illumina RNA PCR Primer (100% over 31bp)
TCTCGCGATTCGGAGGGTTTTTGCCCTGCCGAGGAG1924360.22807979758741695No Hit
TCTGTTACAATGAACGTTCGTTCAAATTTTTGTTTT1211050.14353657261023992No Hit
CTGTTGAGTATGAGATAGTGACTAAAAAAAAAAAAA1196310.14178955218971645No Hit
GCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1076160.12754908383653507Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGTGGGACATGAATTAACAACATTTAAAGTTTGTTA1021400.12105879630411548No Hit
TCGTTTTGTTTCGAAAGTTTGTGTGCAAATAATTGA1012220.11997076051982747No Hit
ACGAGATCCGTGATTGCTTCTACAACCATTTGGACA971630.11515993563047555No Hit
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC930800.11032066536114224Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC858890.10179771838421944Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCTA47700.023.7283211
GATAGGC71250.022.64750527
GCCGAGG371000.022.34134328
GTGGGAC190000.022.27582
CGTGGGA166800.022.1681521
GTGACTA256100.022.14626518
ATAGGCT73250.021.98823528
GACGGCA58450.021.8844411
ACGTAAG79800.021.7615512
CCGAGGA385350.021.59896329
GGCCGCC68100.021.5864961
TTGAGGC91750.021.363130
AGTGACT265900.021.2453717
GATAGTG274050.021.22625714
TGGGACA195650.021.1597633
TAGATCT475000.021.08373530
GTGCGCC48650.021.0095371
ACTAAGT169750.020.89324213
CGTTACC178800.020.8090349
AGGCTTG78050.020.71288530
Produced by FastQC (version 0.11.5)