FastQCFastQC Report
Wed 19 Feb 2020
H2JCLAFX2_n02_herms_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JCLAFX2_n02_herms_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31469921
Sequences flagged as poor quality0
Sequence length36
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1430550.45457692760016777No Hit
GGGATAAAAGCAATATTAATAGAAGTATTTGAATTT796610.25313377812419674No Hit
TCTGTTACAATGAACGTTCGTTCAAATTTTTGTTTT593930.18872942197725887No Hit
GGACGCAAGCAACGTAAGGAGAGAAAGAACAGACAA405400.12882142284373704No Hit
TGGGGAGGATATGGAGGTGGACCATGGGGAGGATAC403140.1281032767765766No Hit
GTCGTGATTACCCGCTGAACTTAAGCATATCATTTA393340.12498919206057112No Hit
GTGCGCAATGGGGGTATGGTCGCCCATATGGAGGAT359680.11429326435233188No Hit
ACGAGATCCGTGATTGCTTCTACAACCATTTGGACA341780.1086052932894239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCGG37750.024.6901341
ACGTAAG75400.024.50288612
GTAAGGA78450.023.6457214
GGGCCTA17300.023.6358661
TTGAGGC72350.023.62961230
GCGCAAT59650.023.3883953
GTGCGCA59000.023.2885761
TAAAAGC136800.023.1524015
GACGGCA44900.023.10211
CGTAAGG80550.023.06652813
ATAGAAG153650.023.01387619
GAAGTAT157850.022.67707622
TGCGCAA57950.022.6365182
AACGTAA83100.022.48528111
CGCAATG62900.022.4319614
AAAGAGC238300.022.39093230
TAGAAGT157700.022.34680220
GGTTACC49800.022.2548857
CCTTACG77750.021.96860922
CAACGTA84900.021.93801110
Produced by FastQC (version 0.11.5)