FastQCFastQC Report
Wed 19 Feb 2020
H2JCLAFX2_n02_herms_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JCLAFX2_n02_herms_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35337512
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1773870.5019793130880296No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT1326140.3752782595447014Illumina RNA PCR Primer (100% over 31bp)
TCTGTTACAATGAACGTTCGTTCAAATTTTTGTTTT704900.1994764090918455No Hit
TCTCGCGATTCGGAGGGTTTTTGCCCTGCCGAGGAG571820.1618167119405577No Hit
GCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT525680.14875976554319953Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC427890.1210866231895443Illumina DpnII expression Sequencing Primer (96% over 30bp)
CTCAAGTAAAGTTATTTGTTGAACAATAAACTTGTT360720.10207849381133569No Hit
CTGTTATTCGTTACCTTTGGAAATAAAATGGTTATT357860.10126915556477208No Hit
CGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC356540.10089561483558888Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCA29600.024.678071
CGCGGCA15950.023.7468761
GACGGCA25300.022.931551
GGCCCCT27250.022.3937171
GGGCCTA26800.022.2649821
GGCCCGG27950.022.20931
CGTTACC91600.022.1512159
TAAAAGC65200.021.9295565
ATGGGCC103200.021.79744510
GATAGGC20200.021.75267627
CGGCACA66650.021.5376223
AAAGAGC106450.021.52662730
GCGAGCC13850.021.487311
ACCTTTG94700.021.3147913
AATGGGC106600.021.2007529
GCGCGCA38400.021.0580271
ATAGGCT21200.020.93882428
GGCGGCA23550.020.870051
GCGGCAC21600.020.8661942
CGTAAGG41500.020.8142313
Produced by FastQC (version 0.11.5)