FastQCFastQC Report
Wed 19 Feb 2020
H2JCLAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JCLAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11409523
Sequences flagged as poor quality0
Sequence length15
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGG1765741.5476019461988024No Hit
AAAAAAAAAAAAAAA250590.2196323194229943No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTT1600.09.0060429
TCCGGCC400.00704243739.0013421
TTGCTAA400.00704243739.0013421
GTATGCG400.00704243739.0013421
TAAGGGC755.793918E-79.0013421
CTGGGGC551.2326235E-49.0013421
AAAGGGC551.2326235E-49.0013421
ACAAGGC400.00704243739.0013421
GGTAGGG400.00704243739.0013421
ACTGGGC504.7334319E-49.0013421
GCGAGGC603.2177766E-59.0013411
TTACAGG1850.08.9990535
AGTTACG1151.4551915E-118.9986983
CGGTGTA551.2365695E-48.9986973
GATCTTT88650.08.9349289
AGGATTT117200.08.9291989
CCATGTT76750.08.9244828
CGTTGTT51350.08.9218348
CTTCTTT54900.08.9158169
ACTAGTT192750.08.9026488
Produced by FastQC (version 0.11.5)