FastQCFastQC Report
Wed 19 Feb 2020
H2JCLAFX2_n01_males.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JCLAFX2_n01_males.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84372225
Sequences flagged as poor quality0
Sequence length15
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTG3046800.361114098863696Illumina Small RNA Adapter 2 (100% over 15bp)
GCTGGAATTCTCGGG1138400.1349259190450412No Hit
CTGGAATTCTCGGGT1007170.11937222231605248No Hit
GTGGAATTCTCGGGT927180.10989161421308968No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCCG1850.09.0024541
ACTGCGG3300.09.0024541
AAACGGC11250.09.0024541
CCGAGGC8550.09.0024541
TTGCGGG4350.09.0024541
TCATCGG2100.09.0024541
CAATCGG2100.09.0024541
TACCGGG3300.09.0024541
CTATGGG2150.09.0024531
AATCCGG1700.09.0024531
AGTCCGG2600.09.0024531
AGTCCGC6950.09.0024531
TAAAGGG2600.09.0024531
TAACGGC8900.09.0024531
ACTTACG1350.09.0024531
GTTCGGC8600.09.0024531
CATAGGG2050.09.0024531
GAACGGG4900.09.0024531
AGTTCGG2300.09.0024531
ATTGGGC13950.09.0024531
Produced by FastQC (version 0.11.5)