FastQCFastQC Report
Wed 19 Feb 2020
H2JCLAFX2_n01_herms_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JCLAFX2_n01_herms_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31469921
Sequences flagged as poor quality0
Sequence length15
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGC2050.09.002751
ACGCGAA1350.09.002751
CCTTCGC2050.09.002751
TCGCCGA2050.09.002751
GCCCCGG1650.09.002751
ACAACCC1350.09.002751
TTTAGCC2700.09.002751
TAGAGGG3800.09.002751
AACCGGC3100.09.002751
GCCTTTA2950.09.002751
TGGAGGG3750.09.002751
GGCAGCC5150.09.002751
AATCCGG658.403347E-69.002751
AGCCGAA1350.09.002751
TTGGCCC1800.09.002751
AGTCCGG1550.09.002751
CTCACCG658.403347E-69.002751
AGTCCCG901.05555955E-89.002751
AAATCGG1350.09.002751
CAAACGG901.05555955E-89.002751
Produced by FastQC (version 0.11.5)