FastQCFastQC Report
Wed 19 Feb 2020
H2JCLAFX2_n01_herms_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JCLAFX2_n01_herms_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35337512
Sequences flagged as poor quality0
Sequence length15
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTG1417590.4011572744566737Illumina Small RNA Adapter 2 (100% over 15bp)
AAAAAAAAAAAAAAA740500.20955068936375598No Hit
GCTGGAATTCTCGGG566380.16027727135968145No Hit
CTGGAATTCTCGGGT471720.13348987331083184No Hit
GTGGAATTCTCGGGT392650.11111421766195649No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCCG952.7866918E-99.0024621
GAATCGG952.7866918E-99.0024621
TCGGGGA4550.09.0024621
AACGGGC4300.09.0024621
GAAAGGC8600.09.0024621
GTAGCGA2150.09.0024621
TGACCGA1850.09.0024621
TCCCGGA1850.09.0024621
ACGTCGC1850.09.0024621
CGTCGCG952.7866918E-99.0024621
TGTAGCC2150.09.0024621
GTCCCCG952.7866918E-99.0024621
GTCCCAA1900.09.0024621
CGGTCGG1850.09.0024621
TCCCCGG952.7866918E-99.0024621
ATTTGGG952.7866918E-99.0024621
ATACCGA1900.09.0024621
GCATAGC3950.09.0024621
TCCGGGG1850.09.0024621
TCACGGC3300.09.0024611
Produced by FastQC (version 0.11.5)