FastQCFastQC Report
Thu 7 Sep 2017
H2J5LBGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2J5LBGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333933771
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGG110260150.068.814456
TGTCGGT110360900.068.76017
TCGGTCT110495700.068.743789
CGGTCTC110524400.068.7080610
GTCGGTC110521800.068.70648
CTCGTAG99726150.068.68526514
CCGGGTG727900.068.6748352
ACGAGGT131350750.068.63508610
GGTCTCG108205900.068.6091711
AGGTCTC130089700.068.5901413
CCACGAG142944350.068.565228
GAGGTCT130309850.068.5571312
CACGAGG131643050.068.546229
GAGCTCT12397900.068.54610412
GTCTCGT101728000.068.53139512
TCCACGA143841950.068.525027
TTCGTGT3881600.068.4336853
TCGTAGG23751550.068.4160515
ACGCGTG2006500.068.403512
TCGTAGA29743700.068.3422815
Produced by FastQC (version 0.11.2)