FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92516867
Sequences flagged as poor quality0
Sequence length25
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT953023110.30107407333627No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA9424341.018661818714635No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT8259850.8927939593976956No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG6401240.6918997808259114No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG5811680.6281751845314866No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT4334900.4685523992073792No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA3911000.4227337270294724No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT3436500.37144578188104876No Hit
TTTTTTTTTTTTTTTTTTTTTTATT2336640.25256367576736033No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC2324980.2513033650393717No Hit
GTGGGGGGGGGGGGGGGGGGGGGGG2322220.2510050410591617No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG1809760.1956140602988642No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1620190.17512374257117894No Hit
TTGGGGGGGGGGGGGGGGGGGGGGG1319960.14267236265145036No Hit
TTTTTTTTTTTTTTTTTTTTTATTT1034120.11177637478796164No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT935150.10107886597586577No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT931360.10066921094507016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG28200.018.0460959
AAGGGGG29950.018.00564610
AAAAGGG26700.017.5324948
GGTAAGG10350.016.14215715
AGTGGGG10900.015.852911
GTTAGGG10750.015.7273246
TAGTGGG9050.014.79202910
GTAAGGT14400.014.63452216
GTGGTGA11650.014.50223413
GGAGGGG10650.014.3867783
GGTGAGG11000.014.32533115
AAGTGGG4000.014.00390710
AAAGGTT16250.013.905313510
GTGGTAA14450.013.26857713
GAGGGGG11600.013.19660954
TAAGGTT22500.013.03682417
AAGGTTG8950.012.93967118
TAAAGGG3600.012.9241388
GTAGGGG8100.012.9036925
TGGTGAG13850.012.74743614
Produced by FastQC (version 0.11.5)