FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9698544
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT350445236.13379492839338No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA5817015.997817816777446No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2505592.58347026110311No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT2243652.3133884838796424No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1667731.7195673907341145No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1495181.541654087458901No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1317641.3585956819910288No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT1066611.0997630159743566No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1063471.0965254165986154No Hit
TTTTTTTTTTTTTTTTTTTTTTATT847760.8741105881460145No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC569040.5867272448318015No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC459790.4740814703732849No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT396220.4085355492535787No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA336160.34660872807299736No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA334580.3449796175590893No Hit
TTTTTTTTTTTTTTTTTTTTTATTT325170.3352771302579026No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT312510.3222236244945633No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT306230.3157484257430806No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC282400.2911777272959735No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA280670.2893939543915045No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT245560.25319264417421833No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC227470.23454035987257468No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT222170.22907562207275647No Hit
TTTTTTTTTTTTTTTTTTTTTTACA217530.22429139879140622No Hit
TTTTTTTTTTTTTTTTTTTTTAATT213860.22050732563568304No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT204650.21101105485524424No Hit
TTTTTTTTTTTTTTTTTTTTTTACC203040.20935101186322402No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT200430.20665988626746445No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG191710.19766884596285794No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT188520.19437969245692963No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT184680.19042033525857077No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT183720.18943049595898107No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT174790.18022292830758926No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA174220.17963521122345788No Hit
TTTTTATTTTTTTTTTTTTTTTTTT171150.17646978762997828No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT168130.17335591816668564No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT168050.17327343155838648No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT163090.16815926184383967No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT161110.16611771828843588No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT157660.16256048330553535No Hit
TTTTTTTTTTTTTTTTTTTTTTATA156090.16094168361766467No Hit
TTTTTTTTATTTTTTTTTTTTTTTT155620.1604570747939072No Hit
TTTTTTATTTTTTTTTTTTTTTTTT150320.15499233699408901No Hit
TTTATTTTTTTTTTTTTTTTTTTTT149580.15422933586732193No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT146410.15096080401346842No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT140770.14514549812837887No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT137210.141474844059067No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT135610.13982511189308416No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC130350.13440161739741555No Hit
TTATTTTTTTTTTTTTTTTTTTTTT127840.13181360006202994No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT125570.12947304255154177No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT124380.12824605425309202No Hit
TTTTTTTATTTTTTTTTTTTTTTTT121820.12560648278751946No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT121100.12486410331282716No Hit
TTTTTTTTTTTTTATTTTTTTTTTT118410.1220904911087685No Hit
TTTTATTTTTTTTTTTTTTTTTTTT116640.12026547490014995No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT112730.11623394191952936No Hit
TTTTTTTTTTTTTTTTTTTTATTTT109760.1131716265864237No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA107390.1107279608155616No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT107120.110449568512552No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT106640.10995464886275715No Hit
TTTTTTTTTTTTTTTTTTTTTACAA104940.10820180843640036No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG101860.10502607401688337No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT98570.10163381225058112No Hit
TTTTTTTTTTTATTTTTTTTTTTTT97800.10083987864570186No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT97530.10056148634269226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCTA1450.019.017261
TGGCGTA706.366463E-1119.017261
TCCCGAA451.6557933E-619.017261
TGGCCTT250.00600274719.017261
TCGCCTA950.019.0172581
TCACCTA401.2763137E-519.0172581
TGGCCTC307.679685E-419.0172581
TGGCCTA2250.019.0172581
TGGCCAA401.2763137E-519.0172581
GCCCTAA1450.019.0065672
GGGCTAA1500.019.0065672
GGCGTTA307.7087857E-419.0065652
GGCCTAA1950.019.0065652
GGCCAAA451.66473E-619.0065652
GCCTATA250.006030893419.0018633
CCGTAAA250.006030893419.0018633
GCGTTAA250.006030893419.0018633
GGGTATA451.668679E-619.0018623
GGCTAAA1600.019.0018623
GCGTAAA801.8189894E-1219.0018623
Produced by FastQC (version 0.11.5)