FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11376879
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT398841435.057189234411304No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA6322585.55739407969444No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2725392.3955515392226636No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT2578172.2661487390346684No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1909181.6781227962431526No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1624131.427570777539253No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1448741.2734072323349839No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT1234121.0847614710501887No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1163441.0226354697101026No Hit
TTTTTTTTTTTTTTTTTTTTTTATT934530.8214291459019649No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC609940.5361224286555214No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC501540.44084146451764145No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT429900.3778716465209835No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA370810.3259329733576317No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT363460.31947250208075517No Hit
TTTTTTTTTTTTTTTTTTTTTATTT361050.31735417068248684No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA356670.3135042571868788No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT348020.30590111752089477No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA311800.2740646182489943No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC307130.26995980180504686No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT300090.26377181299018826No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT251170.22077232253239223No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT250470.22015703955364208No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT247000.21710699393040922No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC245210.21553362745617669No Hit
TTTTTTTTTTTTTTTTTTTTTTACA238810.2099081830790325No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT236960.20828207806376423No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG233370.20512655535845992No Hit
TTTTTTTTTTTTTTTTTTTTTAATT232080.20399267672619176No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT229670.2018743453279234No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT221800.19495680669540388No Hit
TTTTTTTTTTTTTTTTTTTTTTACC220960.1942184671209037No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT216960.1907025643851886No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT211770.1861406805855982No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT205810.18090198550938266No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT203650.1790033980320965No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT194700.1711365656609339No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT194280.1707673958736838No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA188720.16588029107103977No Hit
TTTTTATTTTTTTTTTTTTTTTTTT176950.155534747271198No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT174850.1536888983349476No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT171470.1507179605232683No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT168100.1477558124684283No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT166910.14670983140455304No Hit
TTTTTTTTTTTTTTTTTTTTTTATA162570.14289507693630213No Hit
TTTTTTTTATTTTTTTTTTTTTTTT162560.14288628717946283No Hit
TTTTTTATTTTTTTTTTTTTTTTTT161720.14214794760496266No Hit
TTTATTTTTTTTTTTTTTTTTTTTT157290.13825408532515815No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT154850.13610938465637193No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT152890.13438659231587152No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT144880.12734599708760197No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC141530.12440142854644054No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT138390.12164144489890417No Hit
TTATTTTTTTTTTTTTTTTTTTTTT137270.12065699213290393No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT137150.12055151505083249No Hit
TTTTATTTTTTTTTTTTTTTTTTTT129820.1141086232876345No Hit
TTTTTTTATTTTTTTTTTTTTTTTT129200.11356365836359868No Hit
TTTTTTTTTTTTTTTTTTTTATTTT125310.11014444295311569No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT124400.10934457508074051No Hit
TTTTTTTTTTTTTATTTTTTTTTTT123420.10848317891049031No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT120190.10564408745140033No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA117720.10347301751209624No Hit
TTTTTTTTTTTTTTTTTTTTTACAA117220.10303352967013185No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG114040.10023838699523832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCATA451.6550475E-619.0182151
TGCGCTA1050.019.0182151
TGACCTA603.6816346E-919.0182151
TGTCCTA1100.019.0182151
TGCCGTA401.2757915E-519.0182151
TCGGCTA654.838512E-1019.0182151
TCGCGTA801.8189894E-1219.0182151
TGACATA451.6550475E-619.0182151
TGCCCTC900.019.0182151
TGCCCGA359.873789E-519.0182151
TCGCCAA950.019.0182151
TCCGCTA1100.019.0182151
TGAGCTA307.6771976E-419.0182151
TGTGCTA451.6550475E-619.0182151
TGCGGTA307.6771976E-419.0182151
TGGCCTA5850.019.0182151
TCGGGTA603.6816346E-919.0182151
TCCCCGA401.2757915E-519.0182151
TGCGCAA250.00600106719.0182131
TGCCCTA4000.019.0182131
Produced by FastQC (version 0.11.5)