FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7722012
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT263419434.112793401512455No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3988665.165311838417241No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1870382.4221407581340197No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1697382.198105882249341No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1336891.7312715908755387No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1083291.4028597728156857No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC950201.2305083182983916No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT924601.1973563366645896No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA717920.9297058849429397No Hit
TTTTTTTTTTTTTTTTTTTTTTATT638400.8267275419929417No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC379120.49096012800808914No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC329140.4262360638652206No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT289060.37433249261979906No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT274620.3556327029794826No Hit
TTTTTTTTTTTTTTTTTTTTTATTT245680.31815542374189526No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT235330.30475218116729164No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA224970.2913359885998623No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA211590.27400889819907037No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA205500.2661223525682167No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT196080.25392345932640353No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC193270.2502845113423807No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT187360.24263106558238967No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT173990.22531692517442345No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT168390.21806492919202924No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT164070.2124705322913251No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT157300.2037033871483235No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT156770.2030170375285612No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG155490.2013594384468711No Hit
TTTTTTTTTTTTTTTTTTTTTAATT154860.20054358889885177No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT154400.19994788922886939No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC154160.1996370894010525No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT153970.19939103953736412No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT152230.1971377407856916No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT149250.19327864292363184No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT147710.1912843440284734No Hit
TTTTTTTTTTTTTTTTTTTTTTACA145930.18897924530549812No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT143850.18628564679775167No Hit
TTTTTTTTTTTTTTTTTTTTTTACC138650.1795516505283856No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT133310.1726363543594597No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT128460.16635560783899325No Hit
TTTTTATTTTTTTTTTTTTTTTTTT124710.16149936052935426No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT123430.15984176144766415No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT120570.1561380634995128No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT120020.1554258138940991No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA119140.15428621452543714No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT114920.14882131755299008No Hit
TTTTTTTTATTTTTTTTTTTTTTTT111530.14443126998507644No Hit
TTTTTTATTTTTTTTTTTTTTTTTT110050.14251467104687224No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT109260.14149162161364162No Hit
TTTATTTTTTTTTTTTTTTTTTTTT109180.14138802167103598No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT104720.13561232487077204No Hit
TTTTTTTTTTTTTTTTTTTTTTATA102510.13275037645629142No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT95860.12413863122719829No Hit
TTATTTTTTTTTTTTTTTTTTTTTT91100.1179744346421632No Hit
TTTTTTTATTTTTTTTTTTTTTTTT87850.11376568697380941No Hit
TTTTATTTTTTTTTTTTTTTTTTTT86950.11260018761949606No Hit
TTTTTTTTTTTTTTTTTTTTATTTT85820.11113683843019151No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC85320.11048933878890631No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT84450.10936268941307006No Hit
TTTTTTTTTTTTTATTTTTTTTTTT82740.10714824063987469No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT80550.10431219221104553No Hit
TTTTTTTTTTTTTTTTTTTTTTGGG80420.10414384230431135No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG78750.10198119350241881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA451.6528757E-619.0206571
TCCGCTA552.8083377E-819.0206571
TCACCTA1100.019.0206571
TGGCATA250.005996451219.0206551
TGCGCTA502.1513188E-719.0206551
TCGGCTA250.005996451219.0206551
TCGCGTA307.670272E-419.0206551
TGCCCTC401.2742947E-519.0206551
TGCCCTA1750.019.0206551
TCGCCTA1050.019.0206551
TCGCCAA401.2742947E-519.0206551
TGGGCTA1650.019.0206551
TGGCGTA759.094947E-1219.0206551
TGCGGTA307.670272E-419.0206551
TGGCCTA2600.019.0206551
GCCCTCA359.912236E-519.0084552
GGCATAA250.006018714519.0084552
CGCCTCA250.006018714519.0084552
CGCCTAA1050.019.0084552
GGCGTCA250.006018714519.0084552
Produced by FastQC (version 0.11.5)