FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9197964
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT331948436.089334552733625No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA5261195.71995063255303No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2192162.3833100455709544No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT2124652.3099133677844357No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1542661.6771755140594158No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1462091.589580041844043No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1212111.3178025049891475No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT1024751.1141052519883747No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA916980.9969380180222494No Hit
TTTTTTTTTTTTTTTTTTTTTTATT852950.9273247862244297No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC513020.5577538681386446No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC424270.4612651234555821No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT378780.4118085263216947No Hit
TTTTTTTTTTTTTTTTTTTTTATTT328190.3568072238595411No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT303900.33039920573726966No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT299730.3258655937335697No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA287410.3124713251758759No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA276030.3000990219139801No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA253780.2759088859230151No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT250540.2723863672438814No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC242020.26312344775430735No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT222450.24184700005349008No Hit
TTTTTTTTTTTTTTTTTTTTTAATT212440.23096415685036387No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC205640.2235712164126757No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT205090.2229732579949215No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT202690.22036398489926687No Hit
TTTTTTTTTTTTTTTTTTTTTTACA189900.20645873369367396No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT188680.20513235320338283No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT185390.2015554746680896No Hit
TTTTTTTTTTTTTTTTTTTTTTACC183430.1994245683066383No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT182010.1978807483917093No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG179520.19517362755496762No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT177440.1929122575387336No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT171480.18643256268452454No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT166810.18135535211922987No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT166610.18113791269459198No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT159000.17286434258712038No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT157670.17141837041327843No Hit
TTTTTATTTTTTTTTTTTTTTTTTT150520.16364491098247394No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA150060.1631448003058068No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT143900.15644766602695986No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT140590.15284904354920284No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT138540.1506202894466645No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT134710.14645632446484896No Hit
TTTTTTTTATTTTTTTTTTTTTTTT133880.14555395085260173No Hit
TTTTTTTTTTTTTTTTTTTTTTATA132930.14452111358557176No Hit
TTTTTTATTTTTTTTTTTTTTTTTT132720.14429280218970197No Hit
TTTATTTTTTTTTTTTTTTTTTTTT131840.14333606872129528No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT126990.1380631626738265No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT124390.13523645015353397No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT121270.13184439512918295No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC115350.12540818815990146No Hit
TTATTTTTTTTTTTTTTTTTTTTTT113040.12289676280533388No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT111750.1214942785164195No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT111350.12105939966714373No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT109700.11926552441388115No Hit
TTTTTTTTTTTTTTTTTTTTATTTT109420.11896110921938811No Hit
TTTTATTTTTTTTTTTTTTTTTTTT107390.11675409905931357No Hit
TTTTTTTATTTTTTTTTTTTTTTTT105550.11475365635264499No Hit
TTTTTTTTTTTTTATTTTTTTTTTT102480.11141596118445342No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT98430.10701281283553622No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA95960.1043274359412583No Hit
TTTTTTTTTTTTTTTTTTTTTACAA92760.1008484051470521No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG92240.10028306264299361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTA307.670913E-419.0204751
TCGGCTA359.864528E-519.0204751
TCGCCTC451.6530958E-619.0204751
TCGCCTA850.019.0204751
TGGGCTA1750.019.0204751
TCCGCTA552.8088834E-819.0204751
TGGCGTA451.6530958E-619.0204751
TGTCGTA307.670913E-419.0204751
TGGCCTC307.670913E-419.0204751
TCGGGTA359.864528E-519.0204751
GGCGTAA250.006020845419.0073382
CGCCAAA451.6640661E-619.0073382
CGGGTAA359.916977E-519.0073382
CGGCTAA307.7066425E-419.0073382
CCGCTAA552.831257E-819.0073362
GGGCTAA1450.019.0073362
GGGTAAT451.6681406E-619.002483
CGCTAAA801.8189894E-1219.002483
GGCTTAA401.2848861E-519.002483
GCGTAAA401.2848861E-519.002483
Produced by FastQC (version 0.11.5)