FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9221893
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT337913436.64252014201423No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4988565.409475039452312No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT2229102.417182676051435No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2085422.2613795237051657No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1539811.6697331014358983No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1454661.577398479899951No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1153591.2509253794204727No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT1109041.2026164259333738No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA882910.9574064674140114No Hit
TTTTTTTTTTTTTTTTTTTTTTATT874880.9486989276496702No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC472420.5122809384147051No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC400430.43421670583252264No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT388020.42075959892399534No Hit
TTTTTTTTTTTTTTTTTTTTTATTT341950.3708023938252157No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT326540.35409215873573896No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT302100.3275900078216045No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA276330.2996456367472492No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT260120.28206790080951927No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA247480.2683613874071191No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA238730.25887309687935No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT227330.24651120979174232No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC225450.24447258279834735No Hit
TTTTTTTTTTTTTTTTTTTTTAATT220500.23910492129978086No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT218170.23657832507924348No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT210170.22790331659671173No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT198710.21547636694548505No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT194830.21126898783145717No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT192170.20838454751101534No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC190030.20606398274193813No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT188320.20420969967879696No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT181320.19661906725658168No Hit
TTTTTTTTTTTTTTTTTTTTTTACA180020.1952093783781703No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT179460.1946021277843931No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT178500.19356112676648926No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT171180.18562349400497274No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG169060.18332461675710182No Hit
TTTTTTTTTTTTTTTTTTTTTTACC167460.18158961506059548No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT166060.1800714885761524No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT152830.16572519329816557No Hit
TTTTTATTTTTTTTTTTTTTTTTTT152560.16543241176188012No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT145120.15736465387312562No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT143720.15584652738868254No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT143380.15547783952817495No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA141660.15361271270443064No Hit
TTTTTTATTTTTTTTTTTTTTTTTT136040.1475185192454521No Hit
TTTATTTTTTTTTTTTTTTTTTTTT135340.14675945600323057No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT134220.14554495481567611No Hit
TTTTTTTTATTTTTTTTTTTTTTTT133860.1451545794339622No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT133070.1442979223463122No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT124090.13456022532467032No Hit
TTTTTTTTTTTTTTTTTTTTTTATA123110.13349753678556017No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT121120.13133962842553043No Hit
TTTTTTTTTTTTTTTTTTTTATTTT119180.12923593886851648No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT118020.12797806263854938No Hit
TTATTTTTTTTTTTTTTTTTTTTTT117360.1272623744387405No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT113220.12277305754903034No Hit
TTTTTTTATTTTTTTTTTTTTTTTT111400.12079949311925436No Hit
TTTTATTTTTTTTTTTTTTTTTTTT110410.11972596081954107No Hit
TTTTTTTTTTTTTATTTTTTTTTTT105790.11471614342087899No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC101190.10972801354342324No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT98780.10711466723806057No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT93320.10119397394873265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTA250.005992358619.0229511
TGGCCAA250.005992358619.0229511
TGCCCTA401.2730134E-519.022951
TGGCGTA307.664205E-419.022951
GCGCTAA250.00601682919.0095352
GCCCTAA401.2807823E-519.0095332
GGCGTAA307.70066E-419.0095332
GGCCTAA1150.019.0095332
GCGTAAA359.9350385E-519.0028323
GCCTCAA250.00602909519.002833
CCTCGGG250.00602909519.002833
CGCTAAA250.00602909519.002833
GGCTAAA759.094947E-1219.002833
GCCCCAT250.006037409418.99829717
CCTAAAG250.006037409418.9982974
GCTAAAC552.8467184E-818.9982974
GCTAAAA552.8467184E-818.9982974
CGTAAAA502.1782034E-718.9982974
GCCCGCT250.006037409418.99829717
GGTAAAG250.006037409418.9982974
Produced by FastQC (version 0.11.5)