FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6487837
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT224571034.61415568856No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3300205.086749250944498No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1634742.5196995547206256No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1363112.10102380808889No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1117741.722823800906219No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1003831.5472491062891993No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT821191.2657377181331775No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC808041.245469021493604No Hit
TTTTTTTTTTTTTTTTTTTTTTATT601900.9277360081642002No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA564670.8703517058150506No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC315910.4869265365328999No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC282360.4352143865513267No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT258830.39894652100538286No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT241130.3716647011939418No Hit
TTTTTTTTTTTTTTTTTTTTTATTT240100.3700771150693212No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT218820.33727727746550973No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT182410.2811568786330483No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA175820.2709994101269807No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA174870.2695351316625248No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT162780.2509002615201338No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT161730.24928184848047202No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC155560.23977174519026911No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA154080.23749055347722206No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT150360.2317567472795633No Hit
TTTTTTTTTTTTTTTTTTTTTAATT148680.22916728641610445No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT148210.22844285391263683No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT140600.21671321273946925No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT137570.21204293511073105No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT137100.21131850260726343No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT135870.2094226473322311No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT135650.2090835512667781No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT135490.20883693594644873No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT134120.20672529226612815No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC127030.19579715088403116No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT122630.18901522957497238No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG122610.1889844026599312No Hit
TTTTTTTTTTTTTTTTTTTTTTACA120360.18551637471779886No Hit
TTTTTTTTTTTTTTTTTTTTTTACC114430.17637619440809008No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT114080.1758367233948695No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT111860.17241493582529896No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT106620.16433828408451076No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT106150.1636138515810431No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT103510.15954469879560784No Hit
TTTTTATTTTTTTTTTTTTTTTTTT103290.15920560273015488No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT100630.15510562302967845No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA97620.15046617231598144No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT94920.14630453878542263No Hit
TTTATTTTTTTTTTTTTTTTTTTTT93940.144794019948405No Hit
TTTTTTTTATTTTTTTTTTTTTTTT93700.14442409696791086No Hit
TTTTTTATTTTTTTTTTTTTTTTTT93650.14434702968030794No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT91130.14046283838511975No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT89560.13804292555438738No Hit
TTTTTTTTTTTTTTTTTTTTATTTT82090.12652907278650805No Hit
TTTTTTTTTTTTTTTTTTTTTTATA81740.12598960177328747No Hit
TTATTTTTTTTTTTTTTTTTTTTTT81650.12585088065560215No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT79940.1232151794195816No Hit
TTTTTTTATTTTTTTTTTTTTTTTT75130.11580130635217871No Hit
TTTTATTTTTTTTTTTTTTTTTTTT72610.11191711505699048No Hit
TTTTTTTTTTTTTATTTTTTTTTTT70220.10823329870956994No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC69310.1068306740751964No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG67670.10430286704181996No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT67590.10417955938165524No Hit
TTTTTTTTTTTTTTTTTTTTTTAGT67530.10408707863653172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCTA359.852783E-519.0232851
TGCCCTA401.2727256E-519.0232831
TCGCCTA451.6506074E-619.0232831
GCCCTAA401.28105E-519.0089072
CGCCTAA502.1652158E-719.0089072
GGCCTAA307.7020266E-419.0089072
GGGCTAA250.006017786519.0089072
GGGTATA307.7224214E-419.0014343
GCCTAAA850.019.0014343
GGCTAAA307.7224214E-419.0014343
CCCTAAA1400.019.0014343
GGGTCCC250.00603146919.0014323
CCCTCAA250.00603146919.0014323
CCTAAAC1450.018.9987984
GCTAAAA307.7296305E-418.9987984
CCTCAAC250.006036304418.9987964
GGTATAA250.006036304418.9987964
CTAAACC1100.018.998365
CTAACCC307.7308324E-418.9983585
CTAAACA307.7308324E-418.9983585
Produced by FastQC (version 0.11.5)