FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7243079
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT258458935.68356772030237No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4692496.4785845908901445No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1980962.7349694791400174No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1602982.2131195862974846No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1229551.697551552316356No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1201171.6583693205610488No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1086311.4997903515894278No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA822581.1356772444425913No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT755171.0426090893113273No Hit
TTTTTTTTTTTTTTTTTTTTTTATT679170.9376813369010609No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC464670.6415365620062959No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC387600.5351315372923586No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT345870.4775179174491953No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT265930.3671504894534493No Hit
TTTTTTTTTTTTTTTTTTTTTATTT253840.3504586930502898No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA251710.34751795472616No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA235500.3251379696397071No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA229870.31736503219142026No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC215150.29704218330353704No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT213520.2947917591400011No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT194000.2678418943104169No Hit
TTTTTTTTTTTTTTTTTTTTTAATT183440.25326245923867463No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC182030.2513157733057999No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT173900.2400912650545438No Hit
TTTTTTTTTTTTTTTTTTTTTTACA170370.23521764708075116No Hit
TTTTTTTTTTTTTTTTTTTTTTACC160980.2222535471447985No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT140530.1940196979765097No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT136270.18813822132825003No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG135210.18667475530779107No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA133980.1849765824727302No Hit
TTTTTATTTTTTTTTTTTTTTTTTT126890.17518792767550925No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT122760.169485932709004No Hit
TTTATTTTTTTTTTTTTTTTTTTTT122130.16861613686665575No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT120890.16690415774838296No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT116930.16143686959647963No Hit
TTTTTTATTTTTTTTTTTTTTTTTT115910.16002862870886814No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT115040.15882748206943484No Hit
TTTTTTTTATTTTTTTTTTTTTTTT114690.15834426215701913No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT114380.15791626737745093No Hit
TTTTTTTTTTTTTTTTTTTTTTATA113660.15692221498619577No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT113070.15610764427669502No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT112330.15508597931901613No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT109290.15088886922260547No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT104210.14387527735097186No Hit
TTATTTTTTTTTTTTTTTTTTTTTT103790.14329541345607302No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT103410.1427707746940217No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT95640.13204329263839315No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC95610.13200187378875752No Hit
TTTTATTTTTTTTTTTTTTTTTTTT95310.1315876852924012No Hit
TTTTTTTATTTTTTTTTTTTTTTTT94960.13110446537998552No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT92740.12803947050694875No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT91400.1261894285565572No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT90740.12527821386457333No Hit
TTTTTTTTTTTTTTTTTTTTATTTT90640.12514015103245457No Hit
TTTTTTTTTTTTTTTTTTTTTTCCT90210.12454648085434385No Hit
TTTTTTTTTTTTTTTTTTTTTTACT86800.11983853827909373No Hit
TTTTTTTTTTTTTATTTTTTTTTTT86390.11927248066740678No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT86250.1190791927024405No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT85210.11764333924840528No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT84460.11660786800751449No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA84050.11604181039582752No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG80110.11060213481034792No Hit
TTTTTTTTTTTTTTTTTTTTTACAA79750.11010510861472034No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT79040.10912486250667706No Hit
TTTTTTTTTTATTTTTTTTTTTTTT77460.10694346975920048No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT77280.10669495666138669No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT76930.10621173674897098No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT76440.105535228871589No Hit
TTTTTTTTTTTTTTTTATTTTTTTT76210.10521768435771583No Hit
TTTTTTTTTTTATTTTTTTTTTTTT73120.10095154284524578No Hit
TTTTTTTTTTTTATTTTTTTTTTTT72450.10002652187005001No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCTA250.005992414419.0228521
TGGCCTA307.6642487E-419.0228521
TCCCCTA759.094947E-1219.0228521
GGCCTAA307.7020965E-419.0089262
GGGCTAA250.006017819519.0089242
GGCTAAA307.718929E-419.0027563
GCCTAAA401.2846645E-519.0027543
CCCTAAA801.8189894E-1219.0027543
GCTAAAA359.9502475E-518.9989514
GGTGAGG250.006038255518.9977715
AGTGGGG307.7325606E-418.9977711
GTGAGGT307.7325606E-418.9977716
CCCCGTC502.1786764E-718.9977719
GTAAGGT1000.018.9977716
TGAGGTT359.954993E-518.9977717
TACCCAT307.7325606E-418.9977719
GGTAAGT451.6720023E-618.9977715
GGTAAGG850.018.9977715
TTAAGGT801.8189894E-1218.99776816
AAGGTTG706.366463E-1118.99763918
Produced by FastQC (version 0.11.5)