FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8193903
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT283339934.57935735875809No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4804435.863420643373494No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1984912.422423111428095No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT1978052.4140510328228193No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1452371.7725008460559029No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1262171.5403770339971072No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC1190111.4524335960530652No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT953141.163230758284544No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA814120.993568022467437No Hit
TTTTTTTTTTTTTTTTTTTTTTATT710590.8672179790266006No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC484660.5914885738823122No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC424480.5180437210447817No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT341170.4163705623559371No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT298260.3640023563862057No Hit
TTTTTTTTTTTTTTTTTTTTTATTT273110.33330880289893594No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT271530.3313805398965548No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA254890.3110727573904646No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA249400.30437265366700095No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA246380.30068698640928504No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC240100.2930227511846308No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT204300.24933172872561465No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT201320.24569487825276914No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC186980.2281940608767275No Hit
TTTTTTTTTTTTTTTTTTTTTAATT183520.22397140898543713No Hit
TTTTTTTTTTTTTTTTTTTTTTACA173350.21155974143213557No Hit
TTTTTTTTTTTTTTTTTTTTTTACC170710.20833783363068858No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT170240.20776423640846126No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG164340.20056376064007592No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT160700.19612143321686867No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT153660.1875296790796767No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT148440.18115908865408828No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT145470.1775344423774604No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT142230.17358028280295726No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT141450.1726283554979843No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA141290.17243308835850266No Hit
TTTTTATTTTTTTTTTTTTTTTTTT141220.17234765898497945No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT141130.172237821219021No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT140410.17135911909135365No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT140210.1711150351670016No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT132590.16181543764918865No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT130350.15908169769644576No Hit
TTTATTTTTTTTTTTTTTTTTTTTT126340.15418781501318723No Hit
TTTTTTATTTTTTTTTTTTTTTTTT124460.15189342612427803No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT120930.14758534485946442No Hit
TTTTTTTTATTTTTTTTTTTTTTTT120520.14708497281454272No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT118700.1448638091029391No Hit
TTTTTTTTTTTTTTTTTTTTTTATA118040.14405833215257735No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT114430.13965261731802292No Hit
TTATTTTTTTTTTTTTTTTTTTTTT110880.13532012766077411No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT109050.1330867597529529No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC107100.13070694149052045No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT104040.12697245744793415No Hit
TTTTTTTATTTTTTTTTTTTTTTTT99840.12184669503654119No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT99120.12096799290887382No Hit
TTTTATTTTTTTTTTTTTTTTTTTT98960.1207727257693922No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT98650.12039439568664653No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT97980.11957671454006717No Hit
TTTTTTTTTTTTTTTTTTTTATTTT95680.11676974941001864No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT94740.11562255496556403No Hit
TTTTTTTTTTTTTATTTTTTTTTTT94170.1149269157811607No Hit
TTTTTTTTTTTTTTTTTTTTTTCCT94090.11482928221141987No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG93990.11470724024924385No Hit
TTTTTTTTTTTTTTTTTTTTTTACT89380.1090811057929292No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA88550.10806815750686821No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT86500.10556629728225975No Hit
TTTTTTTTTTTTTTTTTTTTTTAGT84340.10293019089925766No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT83410.10179520065102063No Hit
TTTTTTTTTTTTTTTTATTTTTTTT82890.10116058244770533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCTA654.820322E-1019.0212751
TCCGCTA250.00599535419.0212731
GCCCTAA359.9144774E-519.007922
GGCCTAA552.8301656E-819.007922
GCCTAAA706.366463E-1119.001543
CGCTAAA250.0060314119.0015373
CCTAAAC2300.018.998644
GCTAAAC250.00603672718.998644
GCTAAAA250.00603672718.998644
CACATCC307.731256E-418.99829113
TGGTAAG1650.018.99829114
AGTGGGG451.6716185E-618.99829111
CCCACCG654.8930815E-1018.99829111
CCACCTG451.6716185E-618.99829112
ACCTGCG250.006037365618.99829114
CACCTGC502.1781489E-718.99829113
GTGGTGA950.018.99829113
TGGTGAG950.018.99829114
TGTAAGG401.28730335E-518.99829115
GGTAAGT401.28730335E-518.99829115
Produced by FastQC (version 0.11.5)