FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268983
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT43632934.384148566214044No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA672405.298731346282811No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT324292.5555109879328564No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA270842.134307551795414No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG231991.8281568783821374No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT176741.392768855059524No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC171341.3502150935040107No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT156631.2342954948963067No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA114810.9047402526274978No Hit
TTTTTTTTTTTTTTTTTTTTTTATT103720.8173474349144156No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC64930.5116695810739781No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC58770.46312677159583693No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT45910.36178577648400334No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT44410.3499652871630274No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT42020.33113130751160574No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA38870.3063082799375563No Hit
TTTTTTTTTTTTTTTTTTTTTATTT38090.30016162549064884No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA35500.27975158059643035No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA34810.27431415550878147No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC34460.27155604133388705No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT33820.26651263255693736No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT31130.2453145550413205No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT28620.22553493624422075No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT26560.20930146424341384No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT25730.20276079348580714No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT25660.20220917065082827No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC25530.20118472824301034No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG25380.20000267931091276No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT25320.19952985973807372No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT25310.1994510564759339No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT25190.1985054173302558No Hit
TTTTTTTTTTTTTTTTTTTTTAATT24870.19598371294178094No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT24240.19101910742697104No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT24220.19086150090269138No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT24080.1897582552327336No Hit
TTTTTTTTTTTTTTTTTTTTTTACC24000.1891278291356149No Hit
TTTTTTTTTTTTTTTTTTTTTTACA23990.18904902587347505No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT23630.18621210843644082No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT21250.16745693204715903No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT20970.1652504407072435No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT20850.16430480156156543No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT20770.16367437546444674No Hit
TTTTTATTTTTTTTTTTTTTTTTTT20600.16233472000806945No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT19780.1558728525126026No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA18610.14665287084224138No Hit
TTTTTTATTTTTTTTTTTTTTTTTT18130.1428703142595291No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT18110.1427127077352494No Hit
TTTTTTTTATTTTTTTTTTTTTTTT18010.141924675113851No Hit
TTTTTTTTTTTTTTTTTTTTTTATA17860.14074262618175343No Hit
TTTATTTTTTTTTTTTTTTTTTTTT17850.14066382291961357No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT17330.13656605328834193No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT17290.13625084023978257No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT16020.12624282594802294No Hit
TTATTTTTTTTTTTTTTTTTTTTTT15140.11930813887971706No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC15010.11828369647189915No Hit
TTTTATTTTTTTTTTTTTTTTTTTT14680.11568318882128445No Hit
TTTTTTTATTTTTTTTTTTTTTTTT14210.111979435500712No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG13920.10969414089865663No Hit
TTTTTTTTTTTTTATTTTTTTTTTT13620.10733004303446145No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT13340.10512355169454594No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA13090.10315347014104995No Hit
TTTTTTTTTTTTTTTTTTTTTTAGT12910.10173501142253284No Hit
TTTTTTTTTTTTTTTTTTTTATTTT12700.1000801429175962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAC359.945721E-518.9980284
ACCGCCC250.006036469218.99728214
CCACCGC250.006036469218.99728212
TAAGTTT651.00353645E-817.53664217
AAGTTTT601.4678808E-615.83169118
TAAACCC400.00528064814.2490846
TTACCAA759.679989E-713.93188819
TTAAGTT707.267816E-613.57002116
TTTACAA2250.013.08753219
TTAACCA604.0946875E-412.66535319
TTTAAAC2150.012.3708119
TTTCAAA1001.44355E-712.3487219
TTTCACC550.00307029612.08965519
TTTTGAA1501.4551915E-1112.03208419
CCGCCCC802.868431E-511.87376915
TTTTAGT3800.011.24883419
TTTTCAA4750.010.99885819
AAACCCC856.5950665E-410.0581767
TTTAAGT856.597456E-410.0577815
TTTCCAA1054.1072708E-59.95134919
Produced by FastQC (version 0.11.5)