FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377326
Sequences flagged as poor quality0
Sequence length25
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT21166515.367821416280533No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA301472.18880642636529No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT153511.1145509487223795No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA126170.9160503758732501No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG101080.7338858048130944No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT88720.6441467016523321No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT77210.5605789769451821No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC70890.5146929630312649No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA54660.3968559367934679No Hit
TTTTTTTTTTTTTTTTTTTTTTATT53670.38966809600631946No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC26100.189497620752095No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC24420.17730007274966128No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT23860.17323422341551672No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT23460.1703300453196992No Hit
TTTTTTTTTTTTTTTTTTTTTATTT21690.15747905724570654No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT19540.1418690999806872No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT19160.13911013078966053No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA17030.1236453824294321No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT15610.11333555018927981No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA15350.11144783442699839No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT15350.11144783442699839No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT14690.10665594056889945No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT14610.10607510494973593No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA14430.10476822480661803No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT14030.1018640467108005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAG359.90206E-519.0091192
CGATTCG307.6972396E-419.0091172
AAACGGC359.9490906E-518.9973874
GACTTAG1650.017.27160519
TGGGTGA2150.017.23143819
CTATGCG453.5238845E-516.8969942
TAATAGG453.5326782E-516.8920843
AGTAACT453.5414905E-516.88717814
CGCAAGT402.7621994E-416.6239219
ATACTGA402.7629646E-416.62331614
AACTATG402.76373E-416.62271317
ACTAATA1900.016.5163171
CGGGATT1800.016.36004810
CTAATAT1400.016.293532
TCAAACG350.002163087316.293532
ACCATCG702.5051122E-816.28406518
AGCATAA350.002171499716.2840658
ACGTACC702.5063855E-816.2834745
ATAGTGA350.002172026316.2834745
AATAGGG350.002172026316.2834744
Produced by FastQC (version 0.11.5)