FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299632
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT43479133.45493185763355No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA803716.184135201349305No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA344922.653982050303471No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT287232.2100871631354106No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG225551.7354912775308702No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC211461.6270759722752288No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT172611.3281451980252872No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA148381.1417078065175372No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT129550.9968206384576557No Hit
TTTTTTTTTTTTTTTTTTTTTTATT92490.7116629938321002No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC80390.6185597153655804No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC75090.57777894050008No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA47610.36633447006537234No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT46890.36079444027232327No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT46880.36071749541408643No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC45240.34809853866325235No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA45200.34779075923030517No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA43580.3353256921959447No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT35860.27592426163714034No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC32640.25114801728489294No Hit
TTTTTTTTTTTTTTTTTTTTTTACC32400.2493013406872099No Hit
TTTTTTTTTTTTTTTTTTTTTATTT32110.2470699397983429No Hit
TTTTTTTTTTTTTTTTTTTTTTACA31820.2448385389094759No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT31360.24129907543058343No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG29160.22437120661848892No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT29150.2242942617602521No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA26230.2018263631551085No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT23860.18359043175298853No Hit
TTTTTATTTTTTTTTTTTTTTTTTT22820.17558816649636205No Hit
TTTTTTTTTTTTTTTTTTTTTAATT22590.1738184347569158No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC22480.17297204131631108No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT22200.17081758528568086No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT21610.16627783864971007No Hit
TTTTTTTTTTTTTTTTTTTTTTATA20740.15958363598310907No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT20520.1578908491018996No Hit
TTTTTTATTTTTTTTTTTTTTTTTT20220.15558250335479584No Hit
TTTATTTTTTTTTTTTTTTTTTTTT20200.15542861363832225No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT20120.15481305477242788No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT19980.15373582675711278No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT19420.14942691469585237No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT19150.14734940352345893No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT19080.1468107895158014No Hit
TTTTTTTTATTTTTTTTTTTTTTTT19040.14650301008285424No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT18940.1457335615004863No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT18660.14357910546985608No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT18500.1423479877380674No Hit
TTATTTTTTTTTTTTTTTTTTTTTT17430.1341148879067305No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA17060.13126792815196917No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT16900.13003681042018048No Hit
TTTTTTTATTTTTTTTTTTTTTTTT16050.12349649747005306No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT15810.12164982087237003No Hit
TTTTATTTTTTTTTTTTTTTTTTTT15760.12126509658118606No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT15300.11772563310229356No Hit
TTTTTTTTTTTTTATTTTTTTTTTT14900.11464783877282185No Hit
TTTTTTTTTTTTTTTTTTTTTACAA14850.11426311448163788No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT14840.11418616962340108No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT14410.11087754071921897No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG13570.10441417262732834No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT13370.10287527546259248No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT13370.10287527546259248No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT13350.1027213857461189No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA401.27575095E-519.0158271
TGGGCTA401.27575095E-519.0158271
TGGCCTA950.019.0158271
GCCCTAA401.2838234E-519.0019252
CGCCTAA401.2838234E-519.0019252
GGCCTAA950.019.0019252
CCCCTAA1500.019.0019252
GGGCTAA451.6663598E-619.0019252
GGGGTAT359.930322E-519.0019232
GGGTAAA451.6682025E-618.9997313
GGCTAAA401.2851022E-518.9997313
CCCTCAA250.006032052418.9997313
GGGTATA307.7225355E-418.999733
GCCTAAA1350.018.999733
CGCCCCA359.942059E-518.99916
ACTCCCC307.7245344E-418.99915
TTCCAAA359.942059E-518.99919
CTCCCGC250.00603339318.99899916
CCACCGG250.00603339318.99899912
ACCCACA250.00603339318.99899910
Produced by FastQC (version 0.11.5)