FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231454
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT42801034.756474866296266No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA874617.102254733022914No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA385693.131988689792717No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT243041.9736019372221782No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC219121.779360008575229No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG205721.6705455502194968No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT173311.4073607296740276No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA166031.348243620955391No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT102250.8303192811099724No Hit
TTTTTTTTTTTTTTTTTTTTTTATT89560.7272703649507005No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC86900.705669882918891No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC77200.6269012078404878No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA52900.42957349604613737No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA49470.4017202428998566No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT48440.3933561464740055No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA48270.3919756645396418No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT47540.3860477127038444No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC46500.3776024114583249No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC35170.28559735077396314No Hit
TTTTTTTTTTTTTTTTTTTTTTACC34800.28259277244623027No Hit
TTTTTTTTTTTTTTTTTTTTTTACA34580.2808062664135242No Hit
TTTTTTTTTTTTTTTTTTTTTATTT31680.2572568687096717No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT30610.24856795300514678No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA28960.23516915775985137No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG27910.22664265169466338No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT27420.222663615530909No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT24360.19781494071236116No Hit
TTTTTTTTTTTTTTTTTTTTTAATT23630.1918869888765638No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC23570.19139975995855305No Hit
TTTTTATTTTTTTTTTTTTTTTTTT23250.18880120572916245No Hit
TTTATTTTTTTTTTTTTTTTTTTTT22750.1847409647457396No Hit
TTTTTTTTTTTTTTTTTTTTTTATA22170.18003108520496908No Hit
TTTTTTTTATTTTTTTTTTTTTTTT20000.16240963933691394No Hit
TTTTTTATTTTTTTTTTTTTTTTTT19730.1602171092058656No Hit
TTATTTTTTTTTTTTTTTTTTTTTT18960.1539643380913944No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT18940.15380192845205748No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA18270.14836120553427087No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT17780.14438216937051648No Hit
TTTTATTTTTTTTTTTTTTTTTTTT17740.14405735009184265No Hit
TTTTTTTATTTTTTTTTTTTTTTTT17330.14072795248543593No Hit
TTTTTTTTTTTTTTTTTTTTTACAA16690.13553084402665466No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT16350.13276988015792712No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT15750.12789759097781972No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT15590.12659831386312442No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT15510.12594867530577675No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT15000.12180722950268545No Hit
TTTTTTTTTTTTTATTTTTTTTTTT14870.1207515668469955No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT14730.1196146993716371No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT14670.11912747045362637No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT14540.11807180779793641No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT13840.11238747042114443No Hit
TTTTTTTTTTATTTTTTTTTTTTTT13640.1107633740277753No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT13610.11051975956876993No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT13590.11035734992943301No Hit
TTTTTTTTTTTTTTTTTTTTTACCA13450.1092204824540746No Hit
TTTTTTTTTTTATTTTTTTTTTTTT13200.10719036196236319No Hit
TTTTTTTTTTTTTTTTATTTTTTTT13050.10597228966733634No Hit
TTTTTTTTTTTTATTTTTTTTTTTT12920.10491662701164639No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG12820.10410457881496182No Hit
TTTTTTTTTTTTTTTTTTTTTACCC12580.10215566314291885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA250.00598166119.0272481
TCACCAA250.00598166119.0272481
TGGCCTA451.6451049E-619.0272481
TCCCCTA706.366463E-1119.0272481
CGCCTAA250.006009819519.0117862
GGCCTAA451.6579597E-619.0117862
GCCTAAA706.366463E-1119.0040633
CCCTAAA1000.019.0040633
CCTAACC250.00602959519.0009754
CCTAAAC1200.019.0009754
CCTAAAA900.019.0009754
GCTAAAA502.171164E-719.0009754
CTAACCC307.7272585E-418.9978895
CTAAACC1100.018.9978895
CTAAACA250.00603525518.9978895
CTAAAAC850.018.9978895
CTAAAAA603.7216523E-918.9978895
GTAAACC307.7272585E-418.9978895
TTCCCAA359.949084E-518.99711819
ACCTCCC552.8427166E-818.99711814
Produced by FastQC (version 0.11.5)