FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n02_S23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n02_S23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563396
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT51799633.132744359074735No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA897525.740835975018485No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA388912.4875975120826714No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT344302.2022571376669764No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG265381.697458609335063No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC233651.4945029922041504No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT197291.26193235750891No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA171221.0951799799922732No Hit
TTTTTTTTTTTTTTTTTTTTTTGTT157601.008061936962868No Hit
TTTTTTTTTTTTTTTTTTTTTTATT112710.7209305895627212No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC87370.5588475344698337No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC82310.5264820941079548No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA55390.35429283431708924No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA53970.34521004275308365No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT53080.3395173071953619No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT52500.33580743458471174No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA47740.3053608938490312No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC47300.302546507730607No Hit
TTTTTTTTTTTTTTTTTTTTTGTTT44960.2875790906462598No Hit
TTTTTTTTTTTTTTTTTTTTTATTT38820.24830561163006687No Hit
TTTTTTTTTTTTTTTTTTTTTTCTT36570.2339138644335792No Hit
TTTTTTTTTTTTTTTTTTTTTTACA36460.23321026790397315No Hit
TTTTTTTTTTTTTTTTTTTTTTACC35390.22636619257053237No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG35320.22591844932441943No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC34640.22156894350503645No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT34180.21862663074486566No Hit
TTTTTTTTTTTTTTTGTTTTTTTTT30390.19438453213389315No Hit
TTTTTTTTTTTTTTTTTTTTTTCAA30350.19412867885040003No Hit
TTGTTTTTTTTTTTTTTTTTTTTTT26760.17116584665689305No Hit
TTTTTTTTTTTTTTTTTTTTTAATT26370.16867127714283522No Hit
TTTTTGTTTTTTTTTTTTTTTTTTT25930.16585689102441098No Hit
TTTTTATTTTTTTTTTTTTTTTTTT25060.16029208210843576No Hit
TTTTTTTTTTTTTTTTGTTTTTTTT24550.15702995274389853No Hit
TTTGTTTTTTTTTTTTTTTTTTTTT24020.15363989673761477No Hit
TTTTTTGTTTTTTTTTTTTTTTTTT23900.15287233688713542No Hit
TTTTTTTTTTTTTTTTTTTTTTATA23880.1527444102453889No Hit
TTTTTTTTTTTTGTTTTTTTTTTTT23870.1526804469245156No Hit
TTTTTTTTTTTTTTTTTTTTGTTTT23840.15248855696189575No Hit
TTTTTTTTTTTTTGTTTTTTTTTTT23700.15159307046966988No Hit
TTTTTTTTGTTTTTTTTTTTTTTTT23590.1508894739400638No Hit
TTTTTTTTTTTTTTTTTTTTTTGGT23460.1500579507687112No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC23270.14884264767211888No Hit
TTTTGTTTTTTTTTTTTTTTTTTTT22990.1470516746876671No Hit
TTTTTTATTTTTTTTTTTTTTTTTT22310.14270216886828418No Hit
TTTTTTTTATTTTTTTTTTTTTTTT21600.1381607730862814No Hit
TTTATTTTTTTTTTTTTTTTTTTTT21600.1381607730862814No Hit
TTTTTTTTTTTTTTGTTTTTTTTTT20570.13157255103633372No Hit
TTTTTTTTTTTGTTTTTTTTTTTTT20350.1301653579771216No Hit
TTTTTTTTTTTTTTTTTGTTTTTTT20160.12895005488052932No Hit
TTTTTTTTTTTTTTTTTTTTTTCCA19670.12581585215773866No Hit
TTTTATTTTTTTTTTTTTTTTTTTT18970.12133841969660918No Hit
TTTTTTTTTTTTTTTTTTGTTTTTT18900.12089067645049623No Hit
TTATTTTTTTTTTTTTTTTTTTTTT18840.12050689652525655No Hit
TTTTTTTTTTGTTTTTTTTTTTTTT18350.11737269380246591No Hit
TTTTTTTATTTTTTTTTTTTTTTTT18120.11590153742238052No Hit
TTTTTTTTTTTTTTTTTTTTTTGGG18090.11570964745976067No Hit
TTTTTTTTTTTTTTTTTTTGTTTTT17180.10988898526029234No Hit
TTTTTTTTTTTTTTTTTTTTTACAA17140.10963313197679923No Hit
TTTTTTTTTTTTTTTTTTTTTCTTT16240.10387643309820417No Hit
TTTTTTTTTTTTTATTTTTTTTTTT16200.10362057981471105No Hit
TTTTTTTGTTTTTTTTTTTTTTTTT16160.10336472653121793No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG16120.10310887324772482No Hit
TTTTTTTTTGTTTTTTTTTTTTTTT16050.10266113000161187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCTA1000.019.0199811
TCGCCTA359.8598204E-519.0199811
TGGGCTG359.8598204E-519.0199811
TGGGCTA552.8052455E-819.0199811
TCACCTA359.8598204E-519.0199811
TGGCGTA502.1493724E-719.0199811
TCACATA359.8598204E-519.0199811
TGGCCTA1150.019.0199811
GCCCTAA950.019.0102422
CGCCTAA359.898658E-519.0102422
GGCGTAA451.6598333E-619.0102422
GGGGTGA451.6598333E-619.0102422
GGGCTAA451.6598333E-619.0102422
CACATAA307.6948194E-419.010242
CCGCTAA250.006013347319.010242
GGCCTAA1000.019.010242
GGGTTAA250.00602669919.0029433
GGGTAAT250.00602669919.0029433
GCCTAAA1400.019.0029433
CGCTAAA359.927873E-519.0029433
Produced by FastQC (version 0.11.5)